A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization.
Identifieur interne : 000225 ( Main/Exploration ); précédent : 000224; suivant : 000226A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization.
Auteurs : Andrea Sánchez-Vallet [Pays-Bas, Suisse] ; Hui Tian [Pays-Bas] ; Luis Rodriguez-Moreno [Pays-Bas] ; Dirk-Jan Valkenburg [Pays-Bas] ; Raspudin Saleem-Batcha [Allemagne] ; Stephan Wawra [Allemagne] ; Anja Kombrink [Pays-Bas] ; Leonie Verhage [Pays-Bas] ; Ronnie De Jonge [Pays-Bas] ; H Peter Van Esse [Pays-Bas] ; Alga Zuccaro [Allemagne] ; Daniel Croll [Suisse] ; Jeroen R. Mesters [Allemagne] ; Bart P H J. Thomma [Pays-Bas, Allemagne]Source :
- PLoS pathogens [ 1553-7374 ] ; 2020.
Descripteurs français
- KwdFr :
- Ascomycota (composition chimique), Ascomycota (génétique), Ascomycota (métabolisme), Chitine (composition chimique), Chitine (génétique), Chitine (métabolisme), Cladosporium (composition chimique), Cladosporium (génétique), Hyphae (composition chimique), Hyphae (génétique), Hyphae (métabolisme), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Multimérisation de protéines (MeSH), Protéines fongiques (composition chimique), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Structure quaternaire des protéines (MeSH), Triticum (génétique), Triticum (microbiologie), Triticum (métabolisme).
- MESH :
- composition chimique : Ascomycota, Chitine, Cladosporium, Hyphae, Protéines fongiques.
- génétique : Ascomycota, Chitine, Cladosporium, Hyphae, Maladies des plantes, Protéines fongiques, Triticum.
- microbiologie : Maladies des plantes, Triticum.
- métabolisme : Ascomycota, Chitine, Hyphae, Protéines fongiques, Triticum.
- Multimérisation de protéines, Structure quaternaire des protéines.
English descriptors
- KwdEn :
- Ascomycota (chemistry), Ascomycota (genetics), Ascomycota (metabolism), Chitin (chemistry), Chitin (genetics), Chitin (metabolism), Cladosporium (chemistry), Cladosporium (genetics), Fungal Proteins (chemistry), Fungal Proteins (genetics), Fungal Proteins (metabolism), Hyphae (chemistry), Hyphae (genetics), Hyphae (metabolism), Plant Diseases (genetics), Plant Diseases (microbiology), Protein Multimerization (MeSH), Protein Structure, Quaternary (MeSH), Triticum (genetics), Triticum (metabolism), Triticum (microbiology).
- MESH :
- chemical , chemistry : Chitin, Fungal Proteins.
- chemistry : Ascomycota, Cladosporium, Hyphae.
- genetics : Ascomycota, Chitin, Cladosporium, Fungal Proteins, Hyphae, Plant Diseases, Triticum.
- metabolism : Ascomycota, Chitin, Fungal Proteins, Hyphae, Triticum.
- microbiology : Plant Diseases, Triticum.
- Protein Multimerization, Protein Structure, Quaternary.
Abstract
Plants trigger immune responses upon recognition of fungal cell wall chitin, followed by the release of various antimicrobials, including chitinase enzymes that hydrolyze chitin. In turn, many fungal pathogens secrete LysM effectors that prevent chitin recognition by the host through scavenging of chitin oligomers. We previously showed that intrachain LysM dimerization of the Cladosporium fulvum effector Ecp6 confers an ultrahigh-affinity binding groove that competitively sequesters chitin oligomers from host immune receptors. Additionally, particular LysM effectors are found to protect fungal hyphae against chitinase hydrolysis during host colonization. However, the molecular basis for the protection of fungal cell walls against hydrolysis remained unclear. Here, we determined a crystal structure of the single LysM domain-containing effector Mg1LysM of the wheat pathogen Zymoseptoria tritici and reveal that Mg1LysM is involved in the formation of two kinds of dimers; a chitin-dependent dimer as well as a chitin-independent homodimer. In this manner, Mg1LysM gains the capacity to form a supramolecular structure by chitin-induced oligomerization of chitin-independent Mg1LysM homodimers, a property that confers protection to fungal cell walls against host chitinases.
DOI: 10.1371/journal.ppat.1008652
PubMed: 32574207
PubMed Central: PMC7337405
Affiliations:
Links toward previous steps (curation, corpus...)
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xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="De Jonge, Ronnie" sort="De Jonge, Ronnie" uniqKey="De Jonge R" first="Ronnie" last="De Jonge">Ronnie De Jonge</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Van Esse, H Peter" sort="Van Esse, H Peter" uniqKey="Van Esse H" first="H Peter" last="Van Esse">H Peter Van Esse</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Zuccaro, Alga" sort="Zuccaro, Alga" uniqKey="Zuccaro A" first="Alga" last="Zuccaro">Alga Zuccaro</name><affiliation wicri:level="1"><nlm:affiliation>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne</wicri:regionArea><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author><author><name sortKey="Croll, Daniel" sort="Croll, Daniel" uniqKey="Croll D" first="Daniel" last="Croll">Daniel Croll</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Biology, University of Neuchâtel, Neuchâtel</wicri:regionArea><wicri:noRegion>Neuchâtel</wicri:noRegion></affiliation></author><author><name sortKey="Mesters, Jeroen R" sort="Mesters, Jeroen R" uniqKey="Mesters J" first="Jeroen R" last="Mesters">Jeroen R. Mesters</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Lübeck, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Lübeck</wicri:regionArea><wicri:noRegion>Lübeck</wicri:noRegion><wicri:noRegion>Lübeck</wicri:noRegion><wicri:noRegion>Lübeck</wicri:noRegion></affiliation></author><author><name sortKey="Thomma, Bart P H J" sort="Thomma, Bart P H J" uniqKey="Thomma B" first="Bart P H J" last="Thomma">Bart P H J. Thomma</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne</wicri:regionArea><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32574207</idno><idno type="pmid">32574207</idno><idno type="doi">10.1371/journal.ppat.1008652</idno><idno type="pmc">PMC7337405</idno><idno type="wicri:Area/Main/Corpus">000217</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000217</idno><idno type="wicri:Area/Main/Curation">000217</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000217</idno><idno type="wicri:Area/Main/Exploration">000217</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization.</title><author><name sortKey="Sanchez Vallet, Andrea" sort="Sanchez Vallet, Andrea" uniqKey="Sanchez Vallet A" first="Andrea" last="Sánchez-Vallet">Andrea Sánchez-Vallet</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Group, Institute of Integrative Biology, ETH Zurich, Zurich, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Plant Pathology Group, Institute of Integrative Biology, ETH Zurich, Zurich</wicri:regionArea><wicri:noRegion>Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Hui" sort="Tian, Hui" uniqKey="Tian H" first="Hui" last="Tian">Hui Tian</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Rodriguez Moreno, Luis" sort="Rodriguez Moreno, Luis" uniqKey="Rodriguez Moreno L" first="Luis" last="Rodriguez-Moreno">Luis Rodriguez-Moreno</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Valkenburg, Dirk Jan" sort="Valkenburg, Dirk Jan" uniqKey="Valkenburg D" first="Dirk-Jan" last="Valkenburg">Dirk-Jan Valkenburg</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Saleem Batcha, Raspudin" sort="Saleem Batcha, Raspudin" uniqKey="Saleem Batcha R" first="Raspudin" last="Saleem-Batcha">Raspudin Saleem-Batcha</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Lübeck, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Lübeck</wicri:regionArea><wicri:noRegion>Lübeck</wicri:noRegion><wicri:noRegion>Lübeck</wicri:noRegion><wicri:noRegion>Lübeck</wicri:noRegion></affiliation></author><author><name sortKey="Wawra, Stephan" sort="Wawra, Stephan" uniqKey="Wawra S" first="Stephan" last="Wawra">Stephan Wawra</name><affiliation wicri:level="1"><nlm:affiliation>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne</wicri:regionArea><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author><author><name sortKey="Kombrink, Anja" sort="Kombrink, Anja" uniqKey="Kombrink A" first="Anja" last="Kombrink">Anja Kombrink</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Verhage, Leonie" sort="Verhage, Leonie" uniqKey="Verhage L" first="Leonie" last="Verhage">Leonie Verhage</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="De Jonge, Ronnie" sort="De Jonge, Ronnie" uniqKey="De Jonge R" first="Ronnie" last="De Jonge">Ronnie De Jonge</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Van Esse, H Peter" sort="Van Esse, H Peter" uniqKey="Van Esse H" first="H Peter" last="Van Esse">H Peter Van Esse</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Zuccaro, Alga" sort="Zuccaro, Alga" uniqKey="Zuccaro A" first="Alga" last="Zuccaro">Alga Zuccaro</name><affiliation wicri:level="1"><nlm:affiliation>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne</wicri:regionArea><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author><author><name sortKey="Croll, Daniel" sort="Croll, Daniel" uniqKey="Croll D" first="Daniel" last="Croll">Daniel Croll</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Biology, University of Neuchâtel, Neuchâtel</wicri:regionArea><wicri:noRegion>Neuchâtel</wicri:noRegion></affiliation></author><author><name sortKey="Mesters, Jeroen R" sort="Mesters, Jeroen R" uniqKey="Mesters J" first="Jeroen R" last="Mesters">Jeroen R. Mesters</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Lübeck, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Lübeck</wicri:regionArea><wicri:noRegion>Lübeck</wicri:noRegion><wicri:noRegion>Lübeck</wicri:noRegion><wicri:noRegion>Lübeck</wicri:noRegion></affiliation></author><author><name sortKey="Thomma, Bart P H J" sort="Thomma, Bart P H J" uniqKey="Thomma B" first="Bart P H J" last="Thomma">Bart P H J. Thomma</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University& Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne</wicri:regionArea><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS pathogens</title><idno type="eISSN">1553-7374</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (chemistry)</term><term>Ascomycota (genetics)</term><term>Ascomycota (metabolism)</term><term>Chitin (chemistry)</term><term>Chitin (genetics)</term><term>Chitin (metabolism)</term><term>Cladosporium (chemistry)</term><term>Cladosporium (genetics)</term><term>Fungal Proteins (chemistry)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Hyphae (chemistry)</term><term>Hyphae (genetics)</term><term>Hyphae (metabolism)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Protein Multimerization (MeSH)</term><term>Protein Structure, Quaternary (MeSH)</term><term>Triticum (genetics)</term><term>Triticum (metabolism)</term><term>Triticum (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Ascomycota (composition chimique)</term><term>Ascomycota (génétique)</term><term>Ascomycota (métabolisme)</term><term>Chitine (composition chimique)</term><term>Chitine (génétique)</term><term>Chitine (métabolisme)</term><term>Cladosporium (composition chimique)</term><term>Cladosporium (génétique)</term><term>Hyphae (composition chimique)</term><term>Hyphae (génétique)</term><term>Hyphae (métabolisme)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Multimérisation de protéines (MeSH)</term><term>Protéines fongiques (composition chimique)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Structure quaternaire des protéines (MeSH)</term><term>Triticum (génétique)</term><term>Triticum (microbiologie)</term><term>Triticum (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Chitin</term><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Ascomycota</term><term>Cladosporium</term><term>Hyphae</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Ascomycota</term><term>Chitine</term><term>Cladosporium</term><term>Hyphae</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ascomycota</term><term>Chitin</term><term>Cladosporium</term><term>Fungal Proteins</term><term>Hyphae</term><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Ascomycota</term><term>Chitine</term><term>Cladosporium</term><term>Hyphae</term><term>Maladies des plantes</term><term>Protéines fongiques</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Ascomycota</term><term>Chitin</term><term>Fungal Proteins</term><term>Hyphae</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Ascomycota</term><term>Chitine</term><term>Hyphae</term><term>Protéines fongiques</term><term>Triticum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Protein Multimerization</term><term>Protein Structure, Quaternary</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Multimérisation de protéines</term><term>Structure quaternaire des protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plants trigger immune responses upon recognition of fungal cell wall chitin, followed by the release of various antimicrobials, including chitinase enzymes that hydrolyze chitin. In turn, many fungal pathogens secrete LysM effectors that prevent chitin recognition by the host through scavenging of chitin oligomers. We previously showed that intrachain LysM dimerization of the Cladosporium fulvum effector Ecp6 confers an ultrahigh-affinity binding groove that competitively sequesters chitin oligomers from host immune receptors. Additionally, particular LysM effectors are found to protect fungal hyphae against chitinase hydrolysis during host colonization. However, the molecular basis for the protection of fungal cell walls against hydrolysis remained unclear. Here, we determined a crystal structure of the single LysM domain-containing effector Mg1LysM of the wheat pathogen Zymoseptoria tritici and reveal that Mg1LysM is involved in the formation of two kinds of dimers; a chitin-dependent dimer as well as a chitin-independent homodimer. In this manner, Mg1LysM gains the capacity to form a supramolecular structure by chitin-induced oligomerization of chitin-independent Mg1LysM homodimers, a property that confers protection to fungal cell walls against host chitinases.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32574207</PMID><DateCompleted><Year>2020</Year><Month>08</Month><Day>10</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>10</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1553-7374</ISSN><JournalIssue CitedMedium="Internet"><Volume>16</Volume><Issue>6</Issue><PubDate><Year>2020</Year><Month>06</Month></PubDate></JournalIssue><Title>PLoS pathogens</Title><ISOAbbreviation>PLoS Pathog</ISOAbbreviation></Journal><ArticleTitle>A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization.</ArticleTitle><Pagination><MedlinePgn>e1008652</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.ppat.1008652</ELocationID><Abstract><AbstractText>Plants trigger immune responses upon recognition of fungal cell wall chitin, followed by the release of various antimicrobials, including chitinase enzymes that hydrolyze chitin. In turn, many fungal pathogens secrete LysM effectors that prevent chitin recognition by the host through scavenging of chitin oligomers. We previously showed that intrachain LysM dimerization of the Cladosporium fulvum effector Ecp6 confers an ultrahigh-affinity binding groove that competitively sequesters chitin oligomers from host immune receptors. Additionally, particular LysM effectors are found to protect fungal hyphae against chitinase hydrolysis during host colonization. However, the molecular basis for the protection of fungal cell walls against hydrolysis remained unclear. Here, we determined a crystal structure of the single LysM domain-containing effector Mg1LysM of the wheat pathogen Zymoseptoria tritici and reveal that Mg1LysM is involved in the formation of two kinds of dimers; a chitin-dependent dimer as well as a chitin-independent homodimer. In this manner, Mg1LysM gains the capacity to form a supramolecular structure by chitin-induced oligomerization of chitin-independent Mg1LysM homodimers, a property that confers protection to fungal cell walls against host chitinases.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sánchez-Vallet</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0002-3668-9503</Identifier><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Plant Pathology Group, Institute of Integrative Biology, ETH Zurich, Zurich, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Hui</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rodriguez-Moreno</LastName><ForeName>Luis</ForeName><Initials>L</Initials><Identifier Source="ORCID">0000-0003-2385-8782</Identifier><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Valkenburg</LastName><ForeName>Dirk-Jan</ForeName><Initials>DJ</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saleem-Batcha</LastName><ForeName>Raspudin</ForeName><Initials>R</Initials><Identifier Source="ORCID">0000-0003-3361-823X</Identifier><AffiliationInfo><Affiliation>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Lübeck, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wawra</LastName><ForeName>Stephan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kombrink</LastName><ForeName>Anja</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Verhage</LastName><ForeName>Leonie</ForeName><Initials>L</Initials><Identifier Source="ORCID">0000-0001-6402-3605</Identifier><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Jonge</LastName><ForeName>Ronnie</ForeName><Initials>R</Initials><Identifier Source="ORCID">0000-0001-5065-8538</Identifier><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van Esse</LastName><ForeName>H Peter</ForeName><Initials>HP</Initials><Identifier Source="ORCID">0000-0002-3667-060X</Identifier><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zuccaro</LastName><ForeName>Alga</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0002-8026-0114</Identifier><AffiliationInfo><Affiliation>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Croll</LastName><ForeName>Daniel</ForeName><Initials>D</Initials><Identifier Source="ORCID">0000-0002-2072-380X</Identifier><AffiliationInfo><Affiliation>Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mesters</LastName><ForeName>Jeroen R</ForeName><Initials>JR</Initials><Identifier Source="ORCID">0000-0001-8532-6699</Identifier><AffiliationInfo><Affiliation>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Lübeck, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thomma</LastName><ForeName>Bart P H J</ForeName><Initials>BPHJ</Initials><Identifier Source="ORCID">0000-0003-4125-4181</Identifier><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University& Research, Wageningen, The Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Cologne, Institute for Plant Sciences, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS Pathog</MedlineTA><NlmUniqueID>101238921</NlmUniqueID><ISSNLinking>1553-7366</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>1398-61-4</RegistryNumber><NameOfSubstance UI="D002686">Chitin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002686" MajorTopicYN="N">Chitin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002963" MajorTopicYN="N">Cladosporium</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055503" MajorTopicYN="Y">Protein Multimerization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020836" MajorTopicYN="N">Protein Structure, Quaternary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList><CoiStatement>The authors declare no conflict of interest exists.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>10</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>05</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>07</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>6</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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Ronnie" uniqKey="De Jonge R" first="Ronnie" last="De Jonge">Ronnie De Jonge</name><name sortKey="Kombrink, Anja" sort="Kombrink, Anja" uniqKey="Kombrink A" first="Anja" last="Kombrink">Anja Kombrink</name><name sortKey="Rodriguez Moreno, Luis" sort="Rodriguez Moreno, Luis" uniqKey="Rodriguez Moreno L" first="Luis" last="Rodriguez-Moreno">Luis Rodriguez-Moreno</name><name sortKey="Thomma, Bart P H J" sort="Thomma, Bart P H J" uniqKey="Thomma B" first="Bart P H J" last="Thomma">Bart P H J. Thomma</name><name sortKey="Tian, Hui" sort="Tian, Hui" uniqKey="Tian H" first="Hui" last="Tian">Hui Tian</name><name sortKey="Valkenburg, Dirk Jan" sort="Valkenburg, Dirk Jan" uniqKey="Valkenburg D" first="Dirk-Jan" last="Valkenburg">Dirk-Jan Valkenburg</name><name sortKey="Van Esse, H Peter" sort="Van Esse, H Peter" uniqKey="Van Esse H" first="H Peter" last="Van Esse">H Peter Van Esse</name><name sortKey="Verhage, Leonie" sort="Verhage, Leonie" uniqKey="Verhage L" first="Leonie" last="Verhage">Leonie Verhage</name></country><country name="Suisse"><noRegion><name sortKey="Sanchez Vallet, Andrea" sort="Sanchez Vallet, Andrea" uniqKey="Sanchez Vallet A" first="Andrea" last="Sánchez-Vallet">Andrea Sánchez-Vallet</name></noRegion><name sortKey="Croll, Daniel" sort="Croll, Daniel" uniqKey="Croll D" first="Daniel" last="Croll">Daniel Croll</name></country><country name="Allemagne"><noRegion><name sortKey="Saleem Batcha, Raspudin" sort="Saleem Batcha, Raspudin" uniqKey="Saleem Batcha R" first="Raspudin" last="Saleem-Batcha">Raspudin Saleem-Batcha</name></noRegion><name sortKey="Mesters, Jeroen R" sort="Mesters, Jeroen R" uniqKey="Mesters J" first="Jeroen R" last="Mesters">Jeroen R. Mesters</name><name sortKey="Thomma, Bart P H J" sort="Thomma, Bart P H J" uniqKey="Thomma B" first="Bart P H J" last="Thomma">Bart P H J. Thomma</name><name sortKey="Wawra, Stephan" sort="Wawra, Stephan" uniqKey="Wawra S" first="Stephan" last="Wawra">Stephan Wawra</name><name sortKey="Zuccaro, Alga" sort="Zuccaro, Alga" uniqKey="Zuccaro A" first="Alga" last="Zuccaro">Alga Zuccaro</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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